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In today’s digitally connected world, ensuring the secure and covert transmission of sensitive information has become a critical challenge. Traditional encryption alone reveals the existence of secret communication, inviting targeted attacks. This paper presents Stegno Vault, a web-based secure data transmission system that integrates Least Significant Bit (LSB) steganography, Advanced Encryption Standard (AES) encryption, and Deep Genetic Algorithm (GA) optimization to hide secret messages within digital images, audio, and video files. The system is built on a Java Spring Boot backend, MySQL database, and a responsive HTML/CSS/JavaScript frontend, and is accessible through any standard web browser without local installation. The Genetic Algorithm selects optimal, high-entropy embedding positions in each media file, while AES encryption ensures hidden data remains unreadable even if detected. Role-based access control, OTP-based email verification, BCrypt password hashing, and a dedicated Admin governance panel provide enterprise-grade security. Experimental results confirm PSNR above 50 dB for image steganography and 100% data recovery accuracy across all three media types.